|Year : 2015 | Volume
| Issue : 1 | Page : 34-38
The effect of different cassava (Manihot utilissima) components on liver function of male Wistar rats
Carolyn Damilola Ekpruke1, Silvanus Olu Innih2, Maureen Isoken Ebomoyi1
1 Department of Physiology, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City, Nigeria
2 Department of Anatomy, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City, Nigeria
|Date of Web Publication||17-Jun-2015|
Carolyn Damilola Ekpruke
Department of Physiology, School of Basic Medical Sciences, College of Medical Sciences, University of Benin, Benin City
Source of Support: None, Conflict of Interest: None
Introduction: Liver is the main organ in vertebrates that is responsible for metabolism of substances ingested. Cassava is a rich source of carbohydrate that provides calories for many Nigerians. It is grossly deficient in protein, fat, some minerals and vitamins. The effect of consumption of the different cassava components on the liver function of Wistar rats was studied. Material and Method: Male rats (n=28) with weights between 165g-260g were randomly selected and assigned into three experimental groups and one control group of n=7 per group. The rats in the experimental groups were fed with normal rat chow with inclusion of 50% different cassava components thoroughly mixed with the feeds on a daily basis for eight weeks. The control group received equal amount of normal rat chow daily without the inclusion of any cassava components for the same period. All the rats had access to water ad libitum. The rats were sacrificed after 8 weeks of the experiment. Blood samples were collected for estimation of liver function. Result and Discussion: Average values of aspartate aminotransferase (AST), alanine amino transferase (ALT), alkaline phosphatise (ALP), total protein (TP), albumin (ALB), conjugated bilirubin (CB) and total bilirubin (TB) were recorded for each group and compared among the groups. It was observed that there were no statistically significant differences in the parameters investigated except the level of AST which increased in the experimental group fed with inclusion of 50% popo gari and starch in their diet but decreased in the other experimental group, conjugated bilirubin decreased in the experimental groups and the total bilirubin level decreased statistically in the experimental groups fed normal chow with inclusion of 50% popo gari and gari in their diet but no statistically significant difference in the group with inclusion of 50% starch in their diet when compared with the control group. Conclusion: This percentage used may not have adverse effect on liver function.
Keywords: Gari , liver function, popo gari, starch
|How to cite this article:|
Ekpruke CD, Innih SO, Ebomoyi MI. The effect of different cassava (Manihot utilissima) components on liver function of male Wistar rats. J Exp Clin Anat 2015;14:34-8
|How to cite this URL:|
Ekpruke CD, Innih SO, Ebomoyi MI. The effect of different cassava (Manihot utilissima) components on liver function of male Wistar rats. J Exp Clin Anat [serial online] 2015 [cited 2020 Sep 24];14:34-8. Available from: http://www.jecajournal.org/text.asp?2015/14/1/34/158930
| Introduction|| |
Cassava is a major food crop in Nigeria (FAO, 2001) which is strategically valued for its role in food security, poverty alleviation and as a source of raw materials for agro-allied industries in Nigeria with huge potential for the export market (Egesi, 2007). The presence of cyanogenic glycosides constitutes a major limitation to the use of cassava in both human and animal foods (Tonukari, 2004; Sanguanpong, 2003). Cassava tubers are traditionally processed by a wide range of methods which reduce their toxicity, improve pal ability and convert the perishable fresh root into stable products. This toxic agent present in cassava is a factor that can affect the proper functioning of the body systems depending on the percentage concentration (Gonzalez-Reimers et al., 1994). Cyanide of dietary origin has been implicated in the etiology of various disease conditions (Tylleskar et al., 1992; Banae, et al., 1992; Rosling, 1987). Some abnormalities that border on pathological changes as well as metabolic integrity of the organism under repeated intakes of cassava with high cyanide content does occur. Known cases of acute cyanide poisoning from the consumption of cassava are rare, probably because of preparation process of cassava for consumption can destroy the linamarase and remove much of the free acid however, the possibility of chronic toxicity has not been eliminated (Osuntokun, 1970; Jaffiol et al., 1991; Tylleskar et al., 1992).
Liver is the main body organ responsible for metabolism of the substance ingested by vertebrates and contains enzymes capable of hydrolyzing absorbed linamarin to a metabolite which can dissociate to release cyanide ions (Maduagwu and Umoh, 1986). This subjects the liver to a variety of diseases and disorders. Detection of liver damage is associated with the presence of certain liver enzymes in the body such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) which reside in the body and when the liver is injured, these enzymes spilt into the blood stream, raising the enzyme levels in blood and signaling liver damage. Therefore, increased serum enzyme level is proportional to the extent of tissue damage.
About 20% of cassava component, gari (combination of starch and fibers) fed to albino rats has been reported to cause glucose effect by lowering enzymes activity through reducing cyclic alkaline monophosphate concentration but an increase in mean enzymes concentrations and a decrease mean protein concentration were observed at 80% and 100% of the same cassava component fed to experimental animals (Braide et al., 2011). Overall reduction in the activity of the succinate dehydrogenase and cytochrome C oxidase was observed in albino mice fed on maize (control) and gari based diet for 5 weeks (Ezeji et al., 2009).
The aim of this study was to determine the effect of 50% inclusion of cassava components in rats' diet on the liver function.
| Materials and Methods|| |
The rats were obtained and maintained in the animal house of the Department of Anatomy, School of Basic Medical Sciences, University of Benin, Benin City, Edo state, Nigeria. The animals were fed with grower's mash obtained from Edo feeds and Flour Mill Limited, Edo State, Nigeria.
A total of 28 adult male Wistar rats with weights ranging between 160 g and 265 g were randomly selected for the study and assigned into three experimental groups and a control group. There were seven rats in a group. The cassava components used were cassava fiber (popo gari), cassava starch and grated, roasted cassava tuber (gari) which were obtained from Ekiosa Market Benin-city, Nigeria. The rats in the experimental groups were given the normal rat chow with inclusion of 50% cassava components, respectively. The different cassava components were thoroughly mixed with the feeds and given on a daily basis for 8 weeks. The control group received equal amount of feeds daily without any cassava components added for the same period. All the rats were given water liberally. The rats were sacrificed; blood was drawn out through the aorta after dissecting the rat so as to get enough blood and collected in plain bottles for liver function analysis.
The estimation of total and conjugated bilirubin (CB) was done using Evelyn and Malloy method (Magos, 1960), AST and ALT estimation was done by monitoring the concentration of pyruvate hydrozone formed from 2, 4-dinitrophenylhydrazine (Babson et al., 1966), ALP estimation was done using phenolphthalein monophosphate method (Henry et al., 1974), total protein (TP) estimation was done using biuret method (Tietz, 1995) while estimation of albumin (ALB) was done using Bromocresol green method (Doumas et al., 1971).
Statistical analysis was done using analysis of variance, P = 0.05 or less were taken as significant and mean differences were compared using a multiple comparison test designed by Duncan in 1955. The mean and standard error of the mean for each of the groups of animals were calculated.
| Results|| |
[Table 1] and [Figure 1] show the total bilirubin (TB) and the CB level in the control and experimental groups. The TB in the control group was 0.225 ± 0.13 mg/dl while that of the experimental groups with inclusion of 50% popo gari, gari and starch in their daily diets were 0.125 ± 0.03, 0.150 ± 0.03, 0.225 ± 0.06 mg/dl, respectively. It was observed that there was no statistically significant reduction at P < 0.05 in the experimental groups with the inclusion of 50% starch in their diet. However, there was a statistically significant decrease in TB in experimental groups with the inclusion of 50% popo gari and gari, respectively, in their diet when compared with that of the control group.
|Figure 1: The value of total bilirubin and conjugated bilirubin levels in the control and experimental animals; *means statistically significant at P < 0.05|
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|Table 1: The values of TB, CB, liver enzymes, TP and ALB in both the control and experimental animals recorded in mean±SEM |
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The CB level in the control group was 0.19 ± 0.04 mg/dL while that of the experimental groups with inclusion of 50% popo gari, gari and starch in their daily diets were 0.188 ± 0.04, 0.075 ± 0.01, 0.100 ± 0.04, 0.138 ± 0.04 mg/dL, respectively. The CB in the experimental groups decreased when compared with that of the control group which was statistically significant at P < 0.05.
[Table 1] and [Figure 2] show the AST, ALT, and ALP in the control and experimental groups.
|Figure 2: The value of liver enzymes levels in the control and experimental animals; *means statistically significant at P < 0.05|
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Aspartate aminotransferase level in the control group was 131.25 ± 32.22 IU/L and in the experimental groups with inclusion of 50% popo gari, gari and starch in their diet were 153.75 ± 14.75 IU/L, 92.00 ± 19.14 IU/L and 107.75 ± 11.45 IU/L, respectively. Statistically significant increase was observed in the experimental groups with inclusion of 50% popo gari, while in the experimental group with inclusion of 50% gari and starch in their diet showed a statistically significant decrease when compared to the control group.
Alkaline phosphatase level in the control group was 14.00 ± 1.83 IU/L and in the experimental group, it was 12.50 ± 1.55 IU/L, 16.00 ± 2.55 IU/L and 14.20 ± 1.65 IU/L. There was no statistically significant difference between the level of ALP in the experimental groups and the control groups.
Alanine aminotransferase in the control group was 41.25 ± 4.85 IU/L and in the experimental groups with inclusion of 50% popo gari, gari and starch in their daily diet were 43.75 ± 3.07 IU/L, 37.75 ± 3.57 IU/L, and 29.25 ± 3.68 IU/L, respectively. There was no statistically significant difference between the control and the experimental groups when compared to the control group.
[Table 1] and [Figure 3] show the TP and ALB level in the control and experimental groups.
|Figure 3: The value of total protein and albumin levels in the control and experimental animals; *means statistically significant at P < 0.05|
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The TP level in the control group was 5.20 ± 0.07 g/dL and in the experimental groups with inclusion of 50% popo gari, gari and starch in their diet were 5.27 ± 0.05 g/dL, 4.53 ± 0.24 g/dL and 4.98 ± 0.05 g/dL; respectively. The group with the inclusion of 50% gari in their diet showed a statistically significant decrease while there was no statistically significant difference observed in the other group when compared to the control group.
Albumin level in the control group was 3.10 ± 0.11 g/dL and in the experimental groups with inclusion of 50% popo gari, gari and starch in their daily diet was 2.93 ± 0.10, 2.93 ± 0.09 and 2.90 ± 0.07 g/dL, respectively. This showed no statistically significant difference when compared to the control group.
| Discussion|| |
This study was designed primarily to investigate the effect of ingestion of various cassava components on the liver function. The effect of this diet on the TB, CB, AST, ALT, ALP, TP, and ALB serum level has been investigated. Scientists have reported that dietary composition could have marked effect upon cytochrome P450 mixed function oxidases and conjugation enzymes in animals (Bidlack et al., 1986; Yang et al., 1992). Glucose feeding in both man and microorganism causes profound changes in metabolism including inhibition of induction of several enzymes, stimulation of others, and blockage of glucocorticoids (Melvin and Goldberg, 1975).
Total bilirubin has been reported to be a potent physiologic antioxidant that may provide important protection against atherosclerosis, coronary artery, and inflammation (Yamaguchi et al., 1996; Siow et al., 1999; Stocker et al., 1987), total serum bilirubin level concentrations is directly proportional to the protective factor high-density lipoprotein-cholesterol (Schwertner, et al., 1994; Madhavan et al., 1997). Decrease in TB and CB level in the experimental groups showed that there was no organ damage.
Increased mean enzymes concentrations and decreased mean proteins concentration at 80% and 100% gari diet fed to Albino rats (Braide et al., 2011) which was not in consonance with the finding in this study. The difference in result may be due to the different percentage of cassava component used in the previous study which was 80% and 100% of the diet, while in this study, 50% was used. The level of TP in this study decreased, but was not statistically significant in the experimental group fed gari when compared to the control group. The percentage used has no effect on the liver enzymes except AST as reported by Porikos and Van Itallie in 1983 that diet has the effect upon hepatic enzymes both in animals and in healthy humans. ALP was lowered by reduced food intake (Tuba and Madsen, 1952; Jackson, 1952) and raised by dietary fat (Taylor et al., 1952).
The decrease in AST level may be attributed to the fact that slowly digested carbohydrate diet gives a less rapid flow of glucose into the circulating system and a significantly lowered glucose tolerance curves with a bread diet than with a high sucrose diet (Cohen et al., 2006). ALT is considered most reliable hepatocellular injury because it is solely confined to the liver, unlike AST which is also abundantly present in other body organs such as the kidneys, brain, and hearts (Johnson, 1995).
Increase in serum enzymes indicates cell membrane damage and some organ damage like liver (Joan et al., 1987; Bogusz, 1975). It could either be by the cyanide ion or free radicals generated in the course of its metabolism or metabolism of chemical species within the system. Antioxidant status is affected as in the case of high cassava food intake and low protein food intake, the body tries to detoxify the ingested cyanide using sulfur containing amino acids of the body, protein synthesis is compromised (Okafor et al., 2008). Therefore, increased AST level in the group with inclusion of 50% popo gari in their diet may be due to the increased level of cyanide present when compared to the other cassava components used caused by the difference in fermentation process during preparation.
| Summary and Conclusion|| |
Total bilirubin, CB, and AST showed statistically significant difference, but ALT, ALP, TP, and ALB showed no statistically significant difference in the experimental groups when compared to the control group.
This result is based on the 50% cassava components included in their diet and therefore can be concluded that the inclusion of this percentage of cassava component may not have an adverse effect on the liver function since most of the parameters showed no statistically significant difference. However, further study in this area is recommended using different percentage of cassava components.
| References|| |
Babson L.A., Greeley S.J., Coleman C.M., Philips G.D. (1966). Phenolphthalein monophosphate as a substrate for serum alkaline phosphatase. Clin Chem 12 (8):482-90.
Bidlack W.R., Brown R.C., Mohan C. (1986). Nutritional parameters that alter hepatic drug metabolism, conjugation and toxicity. Fed Processor 45 (2):142-8.
Bogusz M. (1975). The usefulness of the enzymatic tests in acute poisoning. Arch Toxicol 34 (2):159-67.
Braide A.S., Adegoke O.A., Bamigbowu E.O., (2011a). Effect of feeding granulated sugar and gari on some hepatic enzymes in albino rats (Rattus norvegicus
). World J Med Sci 6 (2):91-7.
Cohen P.E., Pollack S.E., Pollard J.W. (2006). Genetic analysis of chromosome pairing, recombination, and cell cycle control during first meiotic prophase in mammals. Endocrinol Rev 27 (4):398-42.
Doumas B.T., Watson W.A., Biggs H.G. (1971). Albumin standards and the measurement of serum albumin with bromocresol green. Clin Chim Acta 31 (1):87.
Duncan D.B. (1955). Multiple ranges and multiple Fisher test. Biometrics 11:1-42.
Ezeji E.U., Obidua O., Kalu I.G., Nwachukwu I.N. (2009). Effect of gari diet on marker enzymes of mice liver mitochondria.
Pak J Nutr 8 (4):414-8.
Food and Agriculture Organisation (FAO). (2001). Strategic Environmental Assessment: An Assessment of the Impact of Cassava Production and Processing on The Environment and Biodiversity Rome. Vol. 5. Food and Agriculture Organisation, Italy, p. 45-6.
Henry R.J., Cannon D.C., Winkelman J.W. (1974). Clinical Chemistry Principles and Techniques. 2 nd
ed. Harper and Row, New York, p. 12-3.
Jackson S.H. (1952). The effect of food ingestion on intestinal and serum alkaline phosphatase in rats. J Biol Chem 98 (2):553.
Joan F.Z., Peter R.P., Philip D.M. (1987). Biochemical test for liver disease. In: Clinical Chemistry in Diagnosis and Treatment. 5 th
ed. Edward Arnold, New York, p. 291-2.
Johnson P.J. (1995). The assessment of hepatic function and investigation of jaundice. In: Marshall, WJ, Bangert SK, editors. Clinical Biochemistry - Metabolic and Clinical Aspects. Churchill Livingstone, New York, p. 217-36.
Madhavan M., Wattigney W.A., Srinivasan S.R., Berenson G.S. (1997). Serum bilirubin distribution and its relation to cardiovascular risk in children and young adults. Atherosclerosis 131 (1):107-13.
Melvin L., Goldberg N. (1975). The glucose effect: Carbohydrate repression of enzyme induction, RNA synthesis and glucocorticoid activity. A role for cyclic AMP and cyclic GMP. J Life Sci 17 (12):1747-54.
Okafor P.N., Anoruo K., Bonire A.O., Maduagwu E.N. (2008). The role of low-protein and cassava-cyanide intake in the aetiology of tropical pancreatitis. Glob J Pharmacol 2 (1):06-10.
Porikos K.P., Van Itallie T.B. (1983). Diet-induced changes in serum transaminase and triglyceride levels in healthy adult men. Role of sucrose and excess calories. Am J Med Sci 75 (4):624-30.
Sanguanpong V. (2003). Hydration and physic-chemical properties of small-particles cassava starch.
J Sci Food Agric 83 (2):123-32.
Schwertner H.A., Jackson W.G., Tolan G. (1994). Association of low serum concentration of bilirubin with increased risk of coronary artery disease. Clin Chem 40 (1):18-23.
Siow R.C., Sato H., Mann G.E. (1999). Heme oxygenase-carbon monoxide signalling pathway in atherosclerosis: Anti-atherogenic actions of bilirubin and carbonmonoxide? Cardiovasc Res 41 (2):385-94.
Stocker R., Yamamoto Y., McDonagh A.F., Glazer A.N., Ames B.N. (1987). Bilirubin is an antioxidant of possible physiological importance. Science 235 (4792):1043-6.
Taylor J.D., Madsen N.B., Tuba J. (1952). The effects of some dietary derivedlipids and fat soluble vitamins on rat serum tributyrinase and alkaline phosphatise. Can J Med Sci 30 (4):308.
Tietz N.W. (1995). Clinical Guide to Laboratory Tests. 3 rd
ed. W.B. Saunders Company, Philadelphia, p. 518-9.
Tonukari J. N. (2004). Cassava and future of starch. Electron J Biotechnol 7 (1):1-8.
Tuba J., Madsen N.B. (1952). The relationship of dietary factors to rat serum alkaline phosphatise. The effect of total food consumption, methionine, choline and vitamin B12 in normal and alloxan diabetic adult rats. Can J Med Sci 30 (1):18.
Yamaguchi T., Terakado M., Horio F., Aoki K., Tanaka M., Nakajima H. (1996). Role of bilirubin as an antioxidant in an ischemia-reperfusion of rat liver and induction of heme oxygenase. Biochem Biophys Res Commun 223 (1):129-35.
Yang C.S., Brady J.F., Hong JY. (1992). Dietary effects on cytochromes P
450, xenobiotic metabolism, and toxicity. FASEB Journal 6 (2):737-44.
[Figure 1], [Figure 2], [Figure 3]