|Year : 2015 | Volume
| Issue : 2 | Page : 63-68
Histological alteration of the pulmonary alveoli, renal cortex and spleen following exposure to open refuse dump site
M Onyije Felix1, E Waritimi Gilbert2, A Atoni Dogood2, O Ijomone Meashack3, U Nwoha Polycarp4
1 Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, College of Health Sciences, Niger Delta University, Wilberfoce Island, Bayelsa State, Nigeria
2 Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Niger Delta University, Wilberfoce Island, Bayelsa State, Nigeria
3 Department of Human Anatomy, Cross River University of Technology, Okuku, Cross River State; Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria
4 Department of Human Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, Niger Delta University, Wilberfoce Island, Bayelsa State; Department of Anatomy and Cell Biology, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria
|Date of Web Publication||19-Feb-2016|
M Onyije Felix
Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, College of Health Sciences, Niger Delta University, Wilberfoce Island, Bayelsa State
Source of Support: None, Conflict of Interest: None
Background: Wastes are mainly unwanted products from domestic and industrial sources, which increases due to accelerated industrialization, urbanization, and population growth. Open refuse dumping is the predominant form of waste disposal method in Nigeria and presents huge environmental and health challenges. Hence, this study investigated the effects of long-term exposure of rats to refuse dump sites on histological and serum analysis in three organs; kidney, lungs, and spleen. Materials and Methods: Twelve Wistar rats of both sexes were divided into two groups made up of 6 rats in each group. Group A was the controls and group B was the experimental. The experimental rats were exposed to refuse dump fume by keeping them in a research hut built in a refuse dump site for 8 months while the control rats were keep in the Department of Human Anatomy, Niger Delta University, Wilberforce Island, Bayelsa State. Results: There was loss of the elastic tissue support for bronchioles, alveolar wall and coalescence of adjacent alveoli as compared with the control. Serum urea concentrations were significantly increased (P < 0.05) in exposed rats (9.08 ± 1.58) compared with control rats (5.00 ± 0.32). Similarly, serum creatinine concentrations were significantly increased (P < 0.05) in exposed rats (106.20 ± 14.94) compared to Control rats (53.67 ± 5.68). All measured serum electrolytes were significantly altered (P < 0.05). Sodium ions (Na+) (90.17 ± 7.68) and bicarbonate ions (HCO3−) (2.33 ± 0.33) were significantly reduced (P < 0.05) while potassium ions (K+) (61.83 ± 6.70) and chloride ions (Cl−) (117.2 ± 3.08) were significantly increased (P < 0.05). Conclusion: Our results revealed histological distortion in experimental animals due to exposure to refuse dump site.
Keywords: Alveolar, kidney, necrosis, refuse dump sites, spleen, waste
|How to cite this article:|
Felix M O, Gilbert E W, Dogood A A, Meashack O I, Polycarp U N. Histological alteration of the pulmonary alveoli, renal cortex and spleen following exposure to open refuse dump site. J Exp Clin Anat 2015;14:63-8
|How to cite this URL:|
Felix M O, Gilbert E W, Dogood A A, Meashack O I, Polycarp U N. Histological alteration of the pulmonary alveoli, renal cortex and spleen following exposure to open refuse dump site. J Exp Clin Anat [serial online] 2015 [cited 2019 Nov 20];14:63-8. Available from: http://www.jecajournal.org/text.asp?2015/14/2/63/177020
| Introduction|| |
Adequate management of solid waste is a worldwide challenge. Management of waste is of huge importance for both public and environmental reasons (Porta et al., 2009). Waste management includes the generation, collection, processing, transportation and disposal of solid waste (Porta et al., 2009). Recycling, sewage treatment, composting, incineration and landfilling are among the foremost methods of waste management (Rushton, 2003). Solid waste may refer to any refuse, litter, or mire from waste handling plant, water supply treatment plant, or air pollution control facility, as well as other cast-off materials, such as solid, liquid, semisolid, or contained gaseous material resulting from industrial, commercial, mining and agriculture operations (RCRA).
In Nigeria however, the predominant method of waste management does not fall within the earlier mention methods, but open dumping of solid waste is most prevalent. Open waste dump sites or refuse dump sites are commonly located in the outskirts of many towns and villages, and sometimes in the vicinity of residential areas. These sites are similar to landfill sites but with little or no sanitary controls.
The practice of living on or near dump sites by scavengers for quick respond to “valuable dumps” is no longer a new trend in the world today especially in developing countries, which has been a concern. Refuse disposal on its part is one of the major environmental problems in most developing countries (Nwigwe, 2008). Historically, dumpsites have been the most common method of unorganized waste disposal and remain so in many places around the world. Most dumpsites are located within the vicinity of living communities and wetlands (Salam et al., 2011).
Most people working and living in or near dump sites are partly unaware of the health hazards associated with it (Gwisai et al., 2014). Scavengers and refuse dump employees work with little or no protective gadgets against health hazards. Most scavengers are self-employed and do not have any consideration for their health as their earnings are virtually spent on foods. It has been reported that little or no attention has been given to the health risks to which scavengers and dump site employees are exposed (Chattopadhyay et al., 2008; Chofqi et al., 2004).
The environment which is nature's gift to man has been abused by pollution both on air, land and water (Alabi et al., 2014). Literature reveals that open dump sites can emit or release obnoxious odors and smoke that could affect the respiratory system (Uchiyama 2014; El-Gammal et al., 2011), cause autoimmune disease (Parks et al., 1999), nonmalignant renal (Steenland and Sanderson, 2001) and cardiac disease (Hnizdo and Vallyathan, 2002). Furthermore, pollution of soil and groundwater may lead to direct contamination of indoor air. These may include cases such as seeping of unstable organic chemicals into basements of nearby residents and contamination of home grown vegetables (Abul, 2010). Studies have linked exposure to refuse sites with increase in birth defects and reproductive disorders as well increase in frequency of cancers (Porta et al., 2009; Rushton 2003). Nevertheless, direct association of exposure to refuse dump sites with serious health problems is still unclear. Hence, this study has evaluated the long-term exposure of rats to refuse dump sites on kidney function tests, serum electrolyte composition and histological changes in three filtering organs; kidney, lungs, and spleen.
| Materials and Methods|| |
Adult Wistar rats of approximately 10 weeks old were obtained from Imo State University, Nigeria. The animals were allowed to acclimatize prior to the start of the experiment.
Dump Site Location
The research was carried out in 2012 in a refuse dump site located along Tombia-Amassoma road in Yenegoa Local Government Area of Bayelsa State.
A total of 12 Wistar rats of both sexes were divided into two groups made up of 6 rats in each group. Group A was the control group and group B was the experimental group. The experimental rats were caged and kept in a research hut build in a refuse dump site for 8 months while the Control rats were caged and keep in the Department of Human Anatomy, Niger Delta University, Wilberforce Island, Bayelsa. All animals were allowed free access to commercially purchased feed and water adlibitum. All animals were handled in accordance with the guidelines for animal research as detailed in the NIH guidelines for the care and use of laboratory animals (NIH Publication 1985).
The rats were sacrificed using anesthesia to induce state of unconsciousness at the end of the 8th month. The lungs, kidneys and spleen were excised, fixed in 10% formal saline, dehydrated in ascending grades of alcohol, impregnated and embedded in paraffin wax. Paraffin sections (5 μm thick) were stained with hematoxylin and eosin method for general histological examination. Slides were viewed under a digital microscope (Leica DM750 with attached ICC50 camera) and digital photomicrographs were taken.
Blood samples were obtained from the rats by cardiac puncture at sacrifice and were kept for 30 min at room temperature. Blood samples were centrifuged at 5000 rpm for 10 min at room temperature to obtain serum. Kidney function markers (serum urea and creatinine) as well as electrolytes composition (sodium, potassium, chloride, and bicarbonate ions) were determined using commercially available assay kits (Randox Laboratories, UK) following standard methods.
Data were expressed as mean ± standard error of the mean and were analyzed using Student's t-test. GraphPad Prism 5 (version 5.03, GraphPad Inc., USA) was the statistical package used for data analysis. Significant difference was considered as P < 0.05.
This study was approved by the Bayelsa State Ministry of Environment in 2012.
| Results|| |
Serum Biochemical Analysis
Serum urea concentrations were significantly increased (P < 0.05) in exposed rats (9.08 ± 1.58) compared to control rats (5.00 ± 0.32). Similarly, serum creatinine concentrations were significantly increased (P < 0.05) in exposed rats (106.20 ± 14.94) compared with control rats (53.67 ± 5.68) [Figure 1] and [Figure 2]. All measured serum electrolytes were significantly altered (P < 0.05) following exposure to refuse dump site [Table 1]. Sodium ions (Na +) (90.17 ± 7.68) and bicarbonate ions (HCO3−) (2.33 ± 0.33) were significantly reduced (P < 0.05) while potassium ions (K +) (61.83 ± 6.70) and chloride ions (Cl −) (117.2 ± 3.08) were significantly increased (P < 0.05).
Histopathology of Lungs
The normal histology of the lungs was seen in the tissue sections of the control animals (CN). A distended thin walled pulmonary artery branch lies next to the bronchioles. The epithelial lining of the bronchioles are clearly demonstrated and well arranged. The alveoli were well illustrated with walls consisting of surface epithelium, supporting tissues and blood vessels. Tissue sections of the experimental animals showed loss of the elastic tissue support for bronchioles, as compared with the control. There was loss of the alveolar wall and coalescence of adjacent alveoli. The walls of the alveoli are remarkably atrophic and thin. Most of the alveolar walls have broken down and merged to form spaces considerably larger than normal alveoli [Figure 3].
|Figure 3: Micrograph of the lungs A section of the lungs of control (CN) rats, bronchioles (b) with its epithelial lining and surrounding smooth muscle layer (arrow). Alveoli (a) are well shown with walls consisting of surface epithelium and supporting tissues. Experimental (EXP) rats showed loss of supporting tissue in the walls of the bronchioles (b). Thin atrophic alveoli (a) resulting in coalescence adjacent alveoli (H and E, ×100 [left] and|
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Histopathology of Kidney
The normal histology of renal cortex was seen in control animals. The renal corpuscles were clearly identified with the glomeruli surrounded by narrow Bowman's spaces. The tubules fill the bulk of the parenchyma between the corpuscles. Occasional interlobar arteries were easily identified. Tissue sections from the experimental animals showed infiltration of fibrous-like materials into the bulk of the parenchyma around the renal corpuscles, the lumen were occluded in most of the tubules. The cytoplasm of the epithelial cells lining the tubules seemed to be swollen. The nuclei of the tubular epithelial cells were markedly reduced and the renal medulla was observed to be disorganized [Figure 4].
|Figure 4: Micrograph of the kidney. Tissue section of the kidney (cortex) of control (CN) rats, glomeruli (G) surrounded by narrow space (arrow). Tissue section of kidney (cortex) from experimental group (EXP) showed infiltration into the parenchyma of the cortex resulted in occluded bowman's spaces (dash arrows) and occlusion of lumen within the tubules. Nuclei of tubular epithelia were markedly reduced (circled) showing necrosis of the cells (H and E, ×100 [left] and H and E, ×400 [rightW)|
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Histopathology of Spleen
The normal spleen histology was observed in control animals. The two distinct functional zones were identified; the lymphoid white pulp and hematogenous red pulp [Figure 5]. The white pulp of the spleen was mainly composed of lymphocytes while the red pulp consists of macrophages, plasma cells and many blood cells (erythrocytes, granulocytes, and platelets). The result from the exposed rats showed an overall decrease in cellularity of red pulp compared to the control. On the other hand, there was an increase in cellularity of white pulp. However, many of the cells appeared paler than normal lymphocytes [Figure 5].
|Figure 5: Micrograph of the spleen. Representative of tissue sections from the spleen of control (CN) and experimental (EXP) rats (H and E, ×400). The two major functional regions were identified as white pulp (W) and red pulp (R). It was observed increase cellularity of white pulp and decrease cellularity of red pulp in exposed compared to control|
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| Discussion|| |
It was well documented that living in close proximity to landfills may pose serious health challenge, and reviews are available (Rushton 2003; Saunders, 2007; Franchini et al., 2004; Vrijheid, 2000). However, the direct association of such habitation and development of illnesses seemed difficult to prove.
Damage to the kidney is associated to the decline in renal function which could lead to renal failure. In the present investigation, decreased renal function is clearly evidenced by significant increase in plasma urea and creatinine concentrations in exposed rats. Increased serum urea may point to reduced re-absorption at the renal epithelium (Adedara et al., 2012). Similarly, increase in serum creatinine reflects impairment in kidneys, especially for glomerular filtration rate (Adedara et al., 2012). Thus the increase in both serum urea and creatine indicate a resulting renal failure in the exposed rats. Furthermore, the present investigation showed significant alteration in Na +, K +, Cl −, and HCO3+. These observations are of toxicological significance and may indicate substantial effects on the ion-dependent processes in exposed rats. Altered serum Na +, K +, Cl −, and HCO3+, have been implicated in development of renal failure and also metabolic changes associated with respiratory disorder (Hsieh et al., 2011; Story et al., 2005). In the result of our kidney sections, there were also infiltration of fibrous-like materials into the bulk of the parenchyma around the renal corpuscle, which suggests chronic inflammation within the kidney. The glomerular tufts within the Bowman's capsule are enlarged as a result of the infiltration, thus closing the narrow bowman's space in most of the renal corpuscle. The infiltration is more striking with the tubules around the glomeruli as the lumen are occluded in most of the tubules. The cytoplasm of the epithelial cells lining the tubules seemed to be swollen and thus completely covering the lumen. The nuclei of the tubular epithelial cells were markedly reduced, suggesting necrosis of the tubular epithelial cells. Also, the renal medulla were observed to be markedly disorganized. This was similar to the results obtained by Chibusi et al. (2012) where it was reported that exposure to municipal landfill leachate caused tubular necrosis and congestion.
As earlier mentioned, dangerous health conditions can be associated with inhaling even small amounts of pollutants. People who live or work near hazardous waste sites or factories that releases cadmium into the air have suffered serious health impairment such as damaged lungs (Amal and Fawzy, 2013). The results for the lungs in the experimental group corroborated with the studies of Reichrtová et al. (1986). Where long-term field exposure of rabbits to a bio-indication station located about 3 km downwind of a disposal site of nickel smelter waste dump revealed histologic abnormalities in the lungs and liver tissues. It was also in line with the study of Zaman (2008) where it was reported that inhaled marble dust damages the cells of the respiratory system. Our findings also agreed with El-Gammal et al. (2011) where there was increase in lymphocytic infiltration. Also, persistent changes included, distortion in epithelial lining of bronchioles and alveoli and presence of areas of degeneration after long time exposure to dust. Our results further corroborated the result obtained by Dungworth et al. (2001), where they reported morphological changes such as lymphatic infiltration, bronchio-alveolar hyperplasia as well as bronchioles and pulmonary fibrosis in the lungs of mice exposed to silica inhalation.
There have been reports on the alteration of the organs by toxins in our environment. Cadmium exposure has been reported to induce atrophy of the proximal renal tubules (Pratap and Wendelaar Bonga, 1993).
| Conclusion|| |
In conclusion, this study has shown that long-term habitation in the vicinity of refuse dump sites resulted in damage to major filtering organs like the kidney, lungs and spleen of rats. Since most of these sites hold any type of waste including municipal, industrial and infectious wastes, they may pose serious health challenges to inhabitants, workers and scavengers that are continuously exposed to them. Hence, it is recommended that more sanitary methods of waste management be implemented and sites of waste disposal be situated far away from residential areas to prevent risk of developing illnesses in humans.
| Acknowledgments|| |
Authors wish to acknowledge the Bayelsa State Ministry of Environment for the approval of this research, and the scavengers at the open dump site for their cooperation.
| References|| |
Abul S. (2010). Environmental and health impact of solid waste disposal at Mangwaneni dumpsite in Manzini: Swaziland. J Sustain Dev Afr 12 (7):64-78.
Adedara I.A., Teberen R., Ebokaiwe A.P., Ehwerhemuepha T., Farombi E.O. (2012). Induction of oxidative stress in liver and kidney of rats exposed to Nigerian bonny light crude oil. Environ Toxicol 27:372-9.
Alabi T.D., Oloyede O.B., Sunmonu T.O., Makinwa T.T. (2014). The biological evaluation of the effects of waste dump in River Ona Apata, Ibadan on the liver, kidney and Haematological Parameters of Albino rats. J Environ Sci Toxicol Food Technol 8 (2):99-103.
Amal I.E., Fawzy I.E. (2013). Histopathology and cytotoxicity as biomarkers in treated rats with cadmium and some therapeutic agent. Saudi J Biol Sci 20 (3):265-80.
Chattopadhyay S., Dutta A., Ray S. (2008). Municipal solid waste management in Kolkata, India. - A review. Waste Manage 29 (4):1449-58.
Chibuisi G.A., Adekunle A.B., Oluwasanmi O.A. (2012). Liver and kidney dysfunction in wistar rats exposed to municipal landfill leachate. 2 (4):150-63.
Chofqi A., Younsi A., Lhadi E.K., Mania J., Mudry J., Veron A. (2004). Environmental impact of an urban landfill on a coastal aquifer (El Jadida, Morocco). Laboratory of environmental techniques and geosciences, faculty of science, El Jadida, Morocco. J Afr Earth Sci 39 (3-5):509-16.
Dungworth D.L., Rittinghausen S., Schwartz L., Harkema J.R., Hayashi Y., Kittel B, et al
. (2001). Respiratory System and Mesothelium. In International Classification of Rodent Tumors: The Mouse WHO, IARC, Springer, Berlin, Germany.
El-Gammal M.I., Ibrahim M.S., Badr E.A., Asker S.A., El-Galad N.M. (2011). Health risk assessment of marble dust at marble workshops. Nat Sci 9 (11):144-54.
Franchini M., Rial M., Buiatti E., Bianchi F. (2004). Health effects of exposure to waste incinerator emissions: A review of epidemiological studies. Ann Ist Super Sanita 40:101-15.
Gwisai R.D., Areola O., Segosebe E. (2014). Respiratory and occupational health problems of scavengers and landfill employees in a Municipal Landfill site in Lobatse, Botswana. J Sustain Dev Afr 16 (1):37-55.
Hnizdo E., Vallyathan V. (2002). Chronic obstructive pulmonary disease due to occupational exposure to silica dust: A review of epidemiological and pathological evidence. Occup Environ Med 60:237-43.
Hsieh M., Wu I., Lee C., Wang S., Wu M. (2011). Higher serum potassium level associated with late stage chronic kidney disease. Chang Gung Med J 34:418-25.
Nwigwe N. (2008). Problems and prospects of refuse disposal in Nigerian urban centres. Int J Nat Appl Sci 4:3.
Parks C.G., Conrad K., Cooper G.S. (1999). Occupational exposure to crystalline silica and autoimmune disease. Environ Health Perspect 107:793-802.
Porta D., Milani S., Lazzarino A.I., Perucci C.A., Forastiere F. (2009). Systematic review of epidemiological studies on health effects associated with management of solid waste. Environ Health 8:60.
Pratap H.B., Wendelaar Bonga S.E. (1993). Effect of ambient and dietary cadmium on pavement ceils, chloride cells, and Na +
ATPase activity in the gills of the freshwater teleost oreochromis mossombicus at normal and high calcium levels in the ambient water. Aquat Toxicol 26:133-50.
Reichrtová E., Takác L., Kranerová J., Bencko V., Sulicová L., Holusa R. (1986). Biomonitoring of environmental pollution hazards from a nickel smelter waste dump. J Hyg Epidemiol Microbiol Immunol 30 (4):359-64.
Rushton L. (2003). Health hazards and waste management. Br Med Bull 68:183-97.
Salam N., Ibrahim M.S., Fatoyinbo F.T. (2011). Dumpsites in Lokoja, Nigeria: A silent pollution zone for underground water. Waste Manage Bioresour Technol 1:21-30.
Saunders P. (2007). A systematic review of the evidence of an increased risk of adverse birth outcomes in populations living in the vicinity of landfill waste disposal sites. In: Mitis F, Martuzzi M, editors. Population Health and Waste Management: Scientific Data and Policy Options. Report of a WHO Workshop Rome, Italy, 29-30 March 2007. WHO, Regional Office for Europe, Copenhagen, 25-7.
Steenland K., Sanderson W.T. (2001). Lung cancer among industrial sand workers exposed to Crystalline Silica. Am J Epidemiol 153:695-703.
Story D.A., Tosolini A., Bellomo R., Leblanc M., Bragantini L., Ronco C. (2005). Plasma acid-base changes in chronic renal failure: A Stewart analysis. Int J Artif Organs 28 (10):961-5.
Uchiyama I. (2013). Chronic health effects of inhalation of dust or sludge. Japan Med Assoc J 56 (2):91-5.
Vrijheid M. (2000). Health effects of residence near hazardous waste landfill sites: A review of epidemiologic literature. Environ Health Perspect 108 Suppl 1:101-12.
Zaman MD. (2008). Article Featured in the March 7, 2008, Online Issue of the “Daily Times,” an English Language Pakistani Newspaper.
[Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5]