Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
  • Users Online: 310
  • Home
  • Print this page
  • Email this page
ORIGINAL ARTICLE
Year : 2019  |  Volume : 18  |  Issue : 1  |  Page : 12-18

Determination of the potentials of aqueous and ethanolic extracts of Buchholzia coriacea in preventing sodium metabisulphite-induced oxidation of some hemoglobin SS erythrocyte membrane proteins


1 Department of Anatomy, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Nigeria
2 Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, Lagos, Nigeria

Correspondence Address:
Dr. Muhammad Nurudeen Nurudeen
Department of Anatomy, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B. 12003, Lagos
Nigeria
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/jeca.jeca_8_19

Rights and Permissions

BACKGROUND: The sustenance of the erythrocyte membrane integrity is crucial to ensuring a continuous lamina flow of erythrocytes through narrow blood vessels. So it is imperative to find a substance to attenuate the oxidation of erythrocytes that cause the membrane structure remodelling. OBJECTIVES: To investigate the hydrating strength and modulatory effect of Buchholzia coriacea seed extracts on erythrocyte membrane proteins degradation. MATERIALS AND METHODS: Aqueous and ethanolic extracts of B. coriacea were obtained following standard procedures. Blood samples (5.00 mL) from five known hemoglobin SS individuals in steady state were subjected to electrophoresis to establish the genotype of the individuals. The osmotic fragility assay was performed with 2.50, 5.00, 10.00 and 20.00 mg/mL of the extracts in the presence of different concentrations of phosphate-buffered saline. The graph of percentage hemolysis was plotted against saline concentration, and the mean corpuscular fragility (MCF) determined. Sodium dodecyl sulfate polyacrylamide gel electrophoresis was also done using the same concentrations of extracts. RESULTS: There was no statistically significant difference (P > 0.01) between the average MCF in all the extract concentration and the control. Protein 4.1 and Ankyrin did not appear on the electrophoretic lanes of all the test samples when compared with the control. However, spectrin and ankyrin bands were visible with regular size and intensity. CONCLUSION: B. coriacea seed extracts possess antihemolytic character. However, it does not have the ability to maintain the erythrocyte membrane model despite its acclaimed antioxidant character.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed121    
    Printed6    
    Emailed0    
    PDF Downloaded37    
    Comments [Add]    

Recommend this journal