Journal of Experimental and Clinical Anatomy

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 14  |  Issue : 1  |  Page : 18--23

Effect of curcumin on the expression of Caspase-3 and Bcl-2 in the spleen of diabetic rats


Hagar A Hashish, Rania N Kamal 
 Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura, Egypt

Correspondence Address:
Dr. Hagar A Hashish
Department of Anatomy and Embryology, Faculty of Medicine, Mansoura University, Mansoura
Egypt

Background: The spleen is the largest lymphoid organ, concerns with immunological reaction and filtration of blood. Diabetes mellitus is a primary disorder of carbohydrate metabolism that cause depletion of oxidant defense system. Curcumin has anti-inflammatory, anti-proliferative, anti-apoptotic, anti-bacterial, anti-cancer and potent antioxidant activities. Aim of the Work: The purpose of this study was to clarify the effect of induced type I diabetes on the spleen of albino rat and whether these changes could be prevented by curcumin. Materials and Methods: Eighteen albino rats were divided randomly into three groups; control nondiabetic rats; untreated diabetic rats and curcumin-treated diabetic rats. After 8 weeks of treatment, animals were sacrificed; spleen was dissected, processed and stained with hematoxylin and eosin and immunohistochemistry for activated Caspase-3 and Bcl-2 . Results: Diabetes caused decrease in the body weight, degeneration of splenocytes, increased Caspase-3, and reduced Bcl-2 activity. Treatment with curcumin decreased the blood glucose level, prevented the loss of body weight and protected the spleen against diabetic induced structural changes. Conclusion: The current results suggested that consumption of curcumin protected the spleen against diabetic induced changes, reduced Caspase-3, and improved Bcl-2 expression. Thus, curcumin may attenuate the pathologic effects observed in the spleen of diabetic rats.


How to cite this article:
Hashish HA, Kamal RN. Effect of curcumin on the expression of Caspase-3 and Bcl-2 in the spleen of diabetic rats.J Exp Clin Anat 2015;14:18-23


How to cite this URL:
Hashish HA, Kamal RN. Effect of curcumin on the expression of Caspase-3 and Bcl-2 in the spleen of diabetic rats. J Exp Clin Anat [serial online] 2015 [cited 2020 Sep 20 ];14:18-23
Available from: http://www.jecajournal.org/article.asp?issn=1596-2393;year=2015;volume=14;issue=1;spage=18;epage=23;aulast=Hashish;type=0