ORIGINAL ARTICLE
Year : 2017 | Volume
: 16 | Issue : 1 | Page : 60--70
Microanatomical evaluation of flavonoid-rich fraction of Musa paradisiaca in Aspirin-induced gastric lesion
Margaret Olutayo Alese1, Stephen Olarinde Adewole2, Oluwole Ojo Alese3
1 Department of Anatomy, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria
2 Department of Anatomy and Cell Biology, College of Health Sciences, Obafemi Awolowo University, Ile Ife, Nigeria
3 Department of Physiology, College of Medicine, Ekiti State University, Ado Ekiti, Nigeria
Correspondence Address:
Margaret Olutayo Alese
Department of Anatomy, College of Medicine, Ekiti State University, Ado Ekiti
Nigeria
Aim: This study investigated the healing effects of a flavonoid-rich fraction of Musa paradisiaca (MP) fruit on the histomorphology and histomorphometry of the gastric corpus in Wistar rats following aspirin-induced gastric lesion. This was with a view to providing information on the alternative therapies of treating gastric ulcers using unripe fruits of MP.
Materials and Methods: Ninety adult male Wistar rats were randomly assigned into 6 groups of 15 rats each. Aspirin at a dose of 400 mg/kg was dissolved in distilled water and administered as a single oral dose to induce gastric lesions in the test groups of rats. After 24 h, flavonoid fraction of MP was administered to groups C, D, and E at graded doses for 21 days, whereas group F rats received omeprazole at 1.8 mg/kg. On days 14, 21, and 28, five rats from each group were sacrificed. The pH value of the gastric contents was determined, gastric tissues were processed using paraffin wax embedding method; sections were stained with hematoxylin and eosin and Alcian blue/PAS. Data were analyzed using descriptive and inferential statistics.
Results: Histomorphological and histomorphometric studies showed a restorative effect of flavonoid fraction of MP in all the treated groups of rats as there was a significant (P = 0.0001) (P = 0.0001) increase in the total mucosal and glandular mucosal layers when compared with the test control. There was a progressive and dose-dependent improvement in staining for mucus-secreting cells in the treated groups as well as a significant (P = 0.0001) increase in a number of cells in the treatment groups when compared with the control.
Conclusion: MP attenuated the deleterious effects of aspirin on the stomach by strengthening mucosa defensive factors.
How to cite this article:
Alese MO, Adewole SO, Alese OO. Microanatomical evaluation of flavonoid-rich fraction of Musa paradisiaca in Aspirin-induced gastric lesion.J Exp Clin Anat 2017;16:60-70
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How to cite this URL:
Alese MO, Adewole SO, Alese OO. Microanatomical evaluation of flavonoid-rich fraction of Musa paradisiaca in Aspirin-induced gastric lesion. J Exp Clin Anat [serial online] 2017 [cited 2021 Jan 23 ];16:60-70
Available from: https://www.jecajournal.org/article.asp?issn=1596-2393;year=2017;volume=16;issue=1;spage=60;epage=70;aulast=Alese;type=0 |
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